Effect of imposed thermal history on post thaw survival of Adipose Derived Adult Stem (ADAS) cells: A parametric study

Document Type

Conference Proceeding

Publication Date

12-1-2005

Abstract

The effect of four thermal parameters on post thaw viability of adipose derived adult stem (ADAS) cells was assessed using a two-level four-parameter (2 4) experimental design. The four thermal parameters studied were: cooling rate (CR), end temperature (ET), hold time (HT), and thawing rate (TR), Several passages, including Passage-0 (P0), Passage-1 (P1), Passage-2 (P2), Passage-3 (P3) and Passage-4 (P4), obtained from the suspensions of adherent cultures of ADAS cells were used for this study [1]. A low and high value was assigned to each parameter [CR: 1 and 40°C/min; ET: -80 and -20°C; HT: 1 and 15 min; and TR: 10 and 200°C/min] in such a way that they enclosed all parameter values possible in a typical freezing process using commercially available controlled rate freezer (CRF). Individual effect of each parameter was determined through the calculation of parameter effect values (E) and any coaction effect of parameters was assessed through the estimation of two parameter interaction effect values (I).

Publication Source (Journal or Book title)

Proceedings of the 2005 Summer Bioengineering Conference

First Page

45

Last Page

46

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