Document Type

Conference Proceeding

Publication Date

1-1-2008

Abstract

Incorporation of spiral continuous flow (CF) polymerase chain reaction (PCR) devices into a 96-well titer plate format will enable highly parallel analyses of many nucleic acids under steady-state temperature control for the realization of a high throughput CFPCR multi-reactor platform. Prior to realization of the full platform, verifying the feasibility of small footprint CFPCR devices, each device confined to a footprint of 8x8 mm2, throughout the manufacturing and amplification processes was essential. Small footprint, CFPCR devices in a CFPCR multi-reactor module were designed and double-side hot embossed on one side of a substrate and structures for thermal isolation on the other to improve thermal management. Successful amplification of 99 bp target DNA fragments from λ-DNA template was demonstrated in the small footprint, 20-and 25-turn CFPCR devices.

Publication Source (Journal or Book title)

Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop

First Page

114

Last Page

117

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