Document Type
Conference Proceeding
Publication Date
1-1-2008
Abstract
Incorporation of spiral continuous flow (CF) polymerase chain reaction (PCR) devices into a 96-well titer plate format will enable highly parallel analyses of many nucleic acids under steady-state temperature control for the realization of a high throughput CFPCR multi-reactor platform. Prior to realization of the full platform, verifying the feasibility of small footprint CFPCR devices, each device confined to a footprint of 8x8 mm2, throughout the manufacturing and amplification processes was essential. Small footprint, CFPCR devices in a CFPCR multi-reactor module were designed and double-side hot embossed on one side of a substrate and structures for thermal isolation on the other to improve thermal management. Successful amplification of 99 bp target DNA fragments from λ-DNA template was demonstrated in the small footprint, 20-and 25-turn CFPCR devices.
Publication Source (Journal or Book title)
Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop
First Page
114
Last Page
117
Recommended Citation
Park, D., Chen, P., You, B., Kim, N., Park, T., Lee, T., Datta, P., Desta, Y., Soper, S., Nikitopoulos, D., & Murphy, M. (2008). Small footprint continuous flow PCR devices for a 96-well CFPCR multi-reactor platform. Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop, 114-117. https://doi.org/10.31438/trf.hh2008.32