A disposable high throughput microfluidic thermal reactor

Document Type

Conference Proceeding

Publication Date

1-1-2009

Abstract

A high throughput microfluidic system including 96 continuous flow (CF) thermal reactors and a multi-zone thermal system was designed and fabricated. Numerical simulation and an infrared camera were used to characterize the temperature distribution and ensure the biochemical performance for assays such as the polymerase chain reaction (PCR). With this microfluidic system, five different DNA fragments were simultaneously amplified from a λ-DNA template and the minimum number of starting copies for successful amplification was determined to be 80. © 2009 CBMS.

Publication Source (Journal or Book title)

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

First Page

639

Last Page

641

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