Temporal dynamics of the multi-omic response to endurance exercise training

Authors

David Amar, Stanford University School of Medicine
Nicole R. Gay, Stanford University
Pierre M. Jean-Beltran, Broad Institute
Dam Bae, University of Iowa Carver College of Medicine
Surendra Dasari, Mayo Clinic
Courtney Dennis, Broad Institute
Charles R. Evans, University of Michigan Medical School
David A. Gaul, Georgia Institute of Technology
Olga Ilkayeva, Duke University School of Medicine
Anna A. Ivanova, Emory University
Maureen T. Kachman, University of Michigan, Ann Arbor
Hasmik Keshishian, Broad Institute
Ian R. Lanza, Mayo Clinic
Ana C. Lira, University of Iowa Carver College of Medicine
Michael J. Muehlbauer, Duke University School of Medicine
Venugopalan D. Nair, Icahn School of Medicine at Mount Sinai
Paul D. Piehowski, Pacific Northwest National Laboratory
Jessica L. Rooney, The University of Vermont
Kevin S. Smith, Stanford University School of Medicine
Cynthia L. Stowe, Wake Forest University School of Medicine
Bingqing Zhao, Stanford University
Natalie M. Clark, Broad Institute
David Jimenez-Morales, Stanford University School of Medicine
Malene E. Lindholm, Stanford University School of Medicine
Gina M. Many, Pacific Northwest National Laboratory
James A. Sanford, Pacific Northwest National Laboratory
Gregory R. Smith, Icahn School of Medicine at Mount Sinai
Nikolai G. Vetr, Stanford University School of Medicine
Tiantian Zhang, Emory University School of Medicine
Jose J. Almagro Armenteros, Stanford University
Julian Avila-Pacheco, Broad Institute
Nasim Bararpour, Stanford University
Yongchao Ge, Icahn School of Medicine at Mount Sinai
Zhenxin Hou, Emory University School of Medicine

Document Type

Article

Publication Date

5-2-2024

Abstract

Regular exercise promotes whole-body health and prevents disease, but the underlying molecular mechanisms are incompletely understood1–3. Here, the Molecular Transducers of Physical Activity Consortium4 profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome and immunome in whole blood, plasma and 18 solid tissues in male and female Rattus norvegicus over eight weeks of endurance exercise training. The resulting data compendium encompasses 9,466 assays across 19 tissues, 25 molecular platforms and 4 training time points. Thousands of shared and tissue-specific molecular alterations were identified, with sex differences found in multiple tissues. Temporal multi-omic and multi-tissue analyses revealed expansive biological insights into the adaptive responses to endurance training, including widespread regulation of immune, metabolic, stress response and mitochondrial pathways. Many changes were relevant to human health, including non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular health and tissue injury and recovery. The data and analyses presented in this study will serve as valuable resources for understanding and exploring the multi-tissue molecular effects of endurance training and are provided in a public repository (https://motrpac-data.org/).

Recommended Citation

Amar, D., Gay, N., Jean-Beltran, P., Bae, D., Dasari, S., Dennis, C., Evans, C., Gaul, D., Ilkayeva, O., Ivanova, A., Kachman, M., Keshishian, H., Lanza, I., Lira, A., Muehlbauer, M., Nair, V., Piehowski, P., Rooney, J., Smith, K., Stowe, C., Zhao, B., Clark, N., Jimenez-Morales, D., Lindholm, M., Many, G., Sanford, J., Smith, G., Vetr, N., Zhang, T., Almagro Armenteros, J., Avila-Pacheco, J., Bararpour, N., Ge, Y., & Hou, Z. (2024). Temporal dynamics of the multi-omic response to endurance exercise training. Retrieved from https://repository.lsu.edu/clinical_research_pubs/153