Bacteriochlorophylls c From Chloropseudomonas ethylicum. Composition and NMR studies of the Pheophorbides and Derivatives
The carbon-13 and proton NMR spectra of the methyl pheophorbides 6, methyl 2-vinylpheophorbides 2, methyl mesopheophorbides 7, and other degradation products from the green sulfur bacterium Chloropseudomonas ethylicum are described and assigned. In order to clarify certain spectra, model chlorins substituted with n-propyl, isobutyl, propenyl, and isobutenyl side chains are synthesized from the chlorophyll b degradation product, rhodin gj trimethyl ester (17). Successful separations of the homologous mixture of pheophorbides, using reverse-phase high-performance liquid chromatography, are detailed; only four major bands (rather than the six obtained from Chlorobium thiosulfatophilum cultures) were observed, and only a minute amount of 4-ethyl-5-methylpheophorbide (band 6) was apparent in the C. ethylicum culture presently being studied. © 1980, American Chemical Society. All rights reserved.
Publication Source (Journal or Book title)
Journal of the American Chemical Society
Smith, K., Bushell, M., Rimmer, J., & Unsworth, J. (1980). Bacteriochlorophylls c From Chloropseudomonas ethylicum. Composition and NMR studies of the Pheophorbides and Derivatives. Journal of the American Chemical Society, 102 (7), 2437-2448. https://doi.org/10.1021/ja00527a048