Activation of the zymogen to urokinase-type plasminogen activator is associated with increased interdomain flexibility

Manja A. Behrens, Aarhus Universitet
Kenneth A. Botkjaer, Aarhus Universitet
Sumit Goswami, KU Leuven
Cristiano L.P. Oliveira, Aarhus Universitet
Jan K. Jensen, Aarhus Universitet
Christine R. Schar, Aarhus Universitet
Paul J. Declerck, KU Leuven
Cynthia B. Peterson, The University of Tennessee, Knoxville
Peter A. Andreasen, Aarhus Universitet
Jan Skov Pedersen, Aarhus Universitet


A key regulatory step for serine proteases of the trypsin clan is activation of the initially secreted zymogens, leading to an increase in activity by orders of magnitude. Zymogen activation occurs by cleavage of a single peptide bond near the N-terminus of the catalytic domain. Besides the catalytic domain, most serine proteases have N-terminal A-chains with independently folded domains. Little is known about how zymogen activation affects the interplay between domains. This question is investigated with urokinase-type plasminogen activator (uPA), which has an epidermal growth factor domain and a kringle domain, connected to the catalytic domain by a 15-residue linker. uPA has been implicated under several pathological conditions, and one possibility for pharmacological control is targeting the conversion of the zymogen pro-uPA to active uPA. Therefore, a small-angle X-ray scattering study of the conformations of pro-uPA and uPA in solution was performed. Structural models for the proteins were derived using available atomic-resolution structures for the various domains. Active uPA was found to be flexible with a random conformation of the amino-terminal fragment domain with respect to the serine protease domain. In contrast, pro-uPA was observed to be rigid, with the amino-terminal fragment domain in a fixed position with respect to the serine protease domain. Analytical ultracentrifugation analysis supported the observed difference between pro-uPA and uPA in overall shape and size seen with small-angle X-ray scattering. Upon association of either of two monoclonal Fab (fragment antigen-binding) fragments that are directed against the catalytic domain of, respectively, pro-uPA and uPA, rigid structures were formed. © 2011 Elsevier Ltd. All rights reserved.