An enzymatic synthesis of specifically radiolabelled derivatives of the common osmolyte, glycine betaine

Gary M. King, University of Maine


A simple, rapid and efficient method has been developed for the synthesis of radiolabelled glycine betaine. Relatively inexpensive 14C- or 3H-labelled choline obtained from commercial sources is oxidized effectively using commercially available choline oxidase. Recoveries of >80% with >95% radiochemical purity are obtained using ion exchange chromatography. The addition of [14C-methyl]glycine betaine to samples from the water column of a hypersaline lake illustrate but one application for studies of methylated amine metabolism. © 1987.