Chromosomal location by fluorescence in situ hybridization of the 28S ribosomal RNA gene of the eastern oyster

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The physical location of the 28S ribosomal RNA gene (28S rDNA) was localized to the short arm of chromosome number 2 of the eastern oyster (Crassostrea virginica) by fluorescence in situ hybridization. The existence of a single locus of the 28S rDNA in the genome of the eastern oyster was concluded based on the findings from metaphase chromosomes prepared from gill, mantle, and embryos and from meiotic chromosome bivalents prepared from ripe gonad tissue. The region of the chromosome occupied by the gene was found to be GC-rich, and the location of probe DNA used to identify the 28S gene corresponded exactly with the location of the nucleolus organizer region. The transcriptional activity of the gene did not vary among different cell types but changed with different phases of mitosis. This study is the first report of physical mapping of a specific gene in mollusks and provides techniques for detection of integration of foreign DNA in the oyster genome.

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Journal of Shellfish Research

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