Superovulation of cattle with a recombinant-DNA bovine follicle stimulating hormone

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A recombinant DNA-derived bovine follicle stimulating hormone (rbFSH) was utilized as a superovulatory agent in mature bovine females. Several aspects of utilization of this product were tested; dose and duration of treatment, repeated usage and a supraoptimal dose. Also, the 'optimal' dose was tested by several different investigators. Doses from 0.5 to 12 mg twice daily (BID) were tested over a range of 3-5 days duration. All doses greater than 0.5 mg BID for 4 days produced more than two ova/embryos (defined as a superovulatory response). A dose of 24 mg given for 3 days was more efficacious than lower doses with equal duration, but larger doses and longer durations of treatment did not produce a greater response (P>0.05). Moreover, when a supraoptimal dose was given, the response was not different (P>0.05) from the 24 mg dose. Embryo production was not altered when individual cows were superovulated more than once with the selected dose nor was subsequent reproductive performance affected. The above studies also demonstrate that a recombinant DNA-derived glycoprotein hormone (i.e. pure follicle stimulating hormone) can elicit a superovulatory response without exogenous luteinizing hormone (LH). The overall performance of rbFSH in these studies was similar to data previously reported for pituitary extracts. © 1993.

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Animal Reproduction Science

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