Immunofluorescence and histochemistry of corneal epithelial flat mounts: Use of EDTA

Document Type

Article

Publication Date

1-1-1981

Abstract

We describe a simple and reliable technique for the preparation of corneal epithelial flat mounts. The technique is based on incubation of corneas or whole globes in an EDTA solution prior to fixation. Corneal epithelial flat mounts prepared by this technique are suitable for study by immunofluorescence or enzyme histochemistry. © 1981 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Publication Source (Journal or Book title)

Current Eye Research

First Page

249

Last Page

253

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